FIG 3 .

Nematode survival and maintenance. (A) Confocal micrograph of 4 S. carpocapsae nematodes isolated in adjacent microfluidic traps. Living nematodes (highlighted with black arrows) display characteristic body curvature and movements associated with healthy nematodes; dead nematodes (highlighted with white arrow) display a straight body posture and disintegration of tissues. X. nematophila bacteria constitutively expressing GFP are colonized in the nematode receptacles. (B) Percentage of nematodes in the microfluidic device isolated and alive (circles), escaped (squares), or dead (triangles) over the course of 112 h at room temperature. The average from three independent experiments is indicated; total numbers of nematodes trapped in the device were 18, 22, and 20, respectively. Error bars indicate standard errors.