FIG 4 .

Osteoclast outgrowth from bone marrow cells of GF SW and conventionalized (CONV-D) mice. Bone marrow cells were flushed from the femurs of 8-week-old GF and CONV-D mice and stimulated for differentiation with RANKL and M-CSF. TRAP⁺ cells were quantitated after 4 days of culture. (a) Representative images of bone marrow cells from mice treated under GF and CONV-D conditions. (b) Total osteoclast (OC) numbers per well were quantitated. The results for individual mice and mean values ± SEM are shown.