FIG 7 .

Dose responses of LASV and LCMV GPC-mediated infection to NH₄Cl in cells ± Lamp1. WT (solid lines, filled circles) and KO (dashed lines, empty boxes) cells were pretreated with NH₄Cl at the indicated concentrations. Cells were then infected in triplicate with (A) LASV or (B) LCMV MLV pseudoviruses (titer determined for ~75,000- to 100,000-RLU signal in mock-treated WT cells). At 24 hpi, cells were lysed and analyzed for firefly luciferase activity. Infection signal was normalized to mock-treated cells, converted to inhibition values, and fitted to a sigmoidal dose-response curve. 50% and 90% inhibition are indicated by red and black dashed lines, respectively. Data shown in panels A and B are from a single experiment that was performed two additional times with similar results. In panel C, the average differences between inhibitory NH₄Cl concentrations for LASV and LCMV in WT and KO cells from the three experiments are shown (ΔmM = IC_WT − IC_KO). See Table 1 for details. *, P < 0.05; **, P < 0.01; and ***, P < 0.001.