FIG 1 .
ATG8 is essential for parasite replication and apicoplast function. (A) Regulation of ATG8 expression by anhydrotetracycline (aTC)-dependent binding of TetR-DOZI repressor. (B) Timing of aTC removal and sample collection during a single replication cycle overlaid with ATG8 expression profile (75). FPKM, fragments per kilobase per million. (C) Western blot showing ATG8 knockdown in the presence or absence of IPP. Equal parasite numbers were loaded per lane. Numbers on the left are molecular masses in kilodaltons. (D) Parasitemia of ACPL-GFP-expressing cultures grown for 4 cycles under the indicated conditions, normalized to culture grown in the presence of aTC, i.e., expressing ATG8. Mean ± standard deviation for 3 biological replicates is shown.