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. 2017 Dec 20;6(12):e116. doi: 10.1038/emi.2017.106

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Screening and selection of HLA-A*0201-restricted CD8 T-cell epitopes for the TSA56 antigen. Human peripheral blood mononuclear cells (PBMCs, 5 × 10⁵ cells per well) were isolated from control individuals with HLA-A2 type (CNT, yellow dots, n=3), recovered patients with non-HLA-A2 type (ST (non-A2), red dots, n=3), or patients with HLA-A2 type (ST (A2), blue dots, n=5) and then stimulated with 17 different epitope peptides (1 μM, amino acid sequences summarized Table 2) in the presence of co-stimulatory anti-CD28 and anti-CD49d monoclonal antibodies at 37 °C for 18 h. Cellular spots were stained with anti-IFN-γ specific antibodies and counted. Positive cellular counts were significantly higher in PBMCs stimulated with peptide #4 and #14 among the 17 predicted peptides (*P<0.05). The number of samples for each group varied (2–5 samples per group) due to the limited availability of PBMCs.