Table 1. List of primers used in this study.

Gene	Primer sequencesa	Product size (bp) (amplified region in nt position)b
scaA	Forward: CGGGATCCGATCCATCAGCTTCATCA	2913 (88–3000)
	Reverse: CGGTCGACTATATCTTCGTCTTTGCC
tsa56	Forward: CGGAATCCGCACCAGGATTTAGAGCA	981 (250–1230)
	Reverse: CGGTCGACTTTACCTTGATTCTTTGC

^aRestriction enzyme sites are italisized.

^bO. tsutsugamushi Boryong strain served as the template.⁴⁴