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. 2017 Dec 6;6(12):e108. doi: 10.1038/emi.2017.96

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Copyright © 2017 The Author(s)

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The in vitro replication of matrix protein 1 (M1) triple mutants after acid treatment. (A) In vitro multiple-step replication of wild-type A/WSN/33 (WSN), M(NLS-88R) and M(NLS-88E) in Madin-Darby canine kidney (MDCK) cells at pH 7.4 without acid bypass. (B) The replication kinetics of pH 5.5 pretreated M1 mutants in MDCK cells with acid bypass. All replication experiments had been repeated multiple times. Data (n=2 replicates/virus/time point) from representative experiments are shown.