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. 2017 Dec 6;6(12):e110. doi: 10.1038/emi.2017.98

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Influenza virus protein microarray pipeline. Recombinant HA is expressed in a baculovirus expression system, purified, characterized and undergoes quality control (A). HAs are arrayed onto an epoxysilane-coated glass slide (Schott) using a Versa 110 spotter (Aurora Biomed) (B). The HAs are covalently bound to the slide and blocked; the arrays are incubated with sera, followed by fluorescently labeled secondary antibodies in a 96-well microarray gasket (Arrayit) (C). The arrays are then imaged (D), spots are automatically detected and their fluorescence is measured (E). Data from microarray imaging are analyzed in GraphPad Prism 7.0 (F). HA, hemagglutinin.