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. 2017 Dec 6;6(12):e110. doi: 10.1038/emi.2017.98

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Copyright © 2017 The Author(s)

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Establishing ELISA and IVPM for mallards. ELISA-IVPM correlation for serial 1:2 dilutions of mAb KB2, reacting to a conformational epitope on NC99 (A) and PR8 (B) H1 HA. The PCC and its P-value are indicated in both panels. (C) Testing of commercial secondary antibodies. Antibodies purchased from Antibodies Online (AbO), KPL (KPL) and Novus Biologicals (Novus) are shown, reacting against serum from a mallard infected with H3N8 and H6N2, binding H3, H6 and H18 HAs in ELISA. (D) Reactivity of different concentrations of pooled sera from mallards infected with H4N5 probed with different concentrations of the AbO secondary antibody in IVPM. The arrayed protein is recombinant H4. enzyme-linked immunosorbent assay, ELISA; influenza virus protein microarray, IVPM; Pearson correlation coefficients, PCC.