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. 2017 Dec 20;2017:7053465. doi: 10.1155/2017/7053465

Figure 6.

Figure 6

The CPC spheroids after spontaneous chondrogenesis are strongly positive for typical markers of cartilage tissue. (a) Hematoxylin and Eosin staining revealed rather rounded cells inside the spheroid. (b) N-cadherin is strongly present; (c–f) extracellular matrix typical markers of cartilage are shown throughout the spheroid area; (c) collagen type II; (d) collagen type VI; (e) aggrecan; (f) chondroitin sulfate. Bar size: 20 μm. (g) Control of nonspecific binding of secondary antibody (immune reaction carried out in the absence of the primary antibody). All reactions were done in the same moment. Bar size: 100 μm; μm: micrometers. (h) qPCR analysis of CPC spheroids showing upregulation of the SOX5 and SOX6 genes comparing to monolayer after 21 days of cell culture (∗∗p < 0.01). The expression of SOX9 gene was not statistically significant (p = 0.05293). Standard errors are shown.