Figure 1.

Laser capture microdissection (LCM) approach for the collection of zonation-based normal human liver tissue. (a) FFPE liver tissue was analyzed with HE staining. Representative histology of a normal human hepatic lobule of the enrolled subject is shown (left), with a solid line that connects PV and CV for the demonstration of zones 1, 2, and 3. The portal triad is within the dotted line and contains PV, HA, BD (right, top). CV is located in the center of the hepatic lobule and can be distinguished from PV by the lack of adjacent HA and BD (left, bottom). (b) Representative image of LCM of the fresh frozen normal human liver tissue stained with Hematoxylin. The selected area of liver tissue, zones-1, 2 and 3 (top), were subjected to LCM-based tissue collection (bottom). (c) Total RNA extracted from each zone via LCM was quality-controlled with a bioanalyzer. The displayed results are from one representation of one subjected enrolled in this study. (d) The quality and quantity control of the cDNA library synthesized with a PCR approach using polyA tailed RNA extracted from each zone is shown. The displayed result is from one representation of zone-1 cDNA library obtained from one subject enrolled in this study. *: indicates signal from internal control. PV: portal vein, HA: hepatic artery, BD: bile duct, CV: central vein.