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. 2017 Dec 20;2017:3035184. doi: 10.1155/2017/3035184

Figure 3.

Figure 3

IGFBP-2 stimulates glucose uptake in a PI3K-dependent manner. (a) Insulin, IGF-1, and IGFBP-2 increase the phosphorylation of PI3K. 3T3-L1 cells were cultured and differentiated in 24-well plates for glucose uptake assay and in 6-well plates for Western blotting analysis. The results are presented as mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 versus control. (b and c) The PI3K inhibitors, LY294002 and wortmannin (black bars), significantly reduce basal as well as insulin, IGF-1, and IGFBP-2-induced glucose uptake (white bars). Differentiated 3T3-L1 adipocytes were incubated with 100 μM LY294002 for 1 h or 200 nM wortmannin for 30 min before treatment with insulin, IGF-1, or IGFBP-2 for 30 min. The results are presented as mean ± SEM. ∗∗∗P < 0.001. Each experiment was performed with three technical replicates and total number of experiments was three.