Expression of bacterial factors involved in biofilm production (A–E). After treatment of the bacteria with GLY (0, 5 and 10 mg/mL) for 3 h, mRNA expression levels for bacterial factors rhlR (B) and algA (E) were significantly elevated at 5 mg/mL. Genes rhlI, pslA and pelA were elevated, but not significant compared with control. At 10 mg/mL, bacterial factors pslA (A), rhlI (C) and algA (E) mRNA expression levels were significantly reduced; rhlR (B) and pelA (D) mRNA levels were also reduced, but not significant. Data are mean ± SEM analyzed using a two tailed student’s t test. n = 3/group/time.