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. 2017 Dec 5;6(4):60. doi: 10.3390/plants6040060

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Detection of nuclei and callose-rich sieve plates in green fluorescent protein (GFP)-conducting cells in a 1-cm-diameter tubercle. (A–C) 4′6-diamidino-2-phenylindole (DAPI) staining of haustorial cells; (A) GFP, (B) DAPI, and (C) merged. (D–F) DAPI staining of protrusion cells; (D) GFP, (E) DAPI, and (F) merged. White arrows indicate nuclei. (G–I) Aniline Blue staining of haustorial cells; (G) GFP, (H) Aniline Blue, and (I) merged. (J–L) Aniline Blue staining of protrusion cells; (J) GFP, (H) Aniline Blue, and (I) merged. White triangles indicate sieve plates. Scale bar: 50 μm. (M) Percentage of nucleated GFP-conducting cells to all GFP-conducting cells. Ha, haustorium; Pr, protrusion. The means and standard deviations of six and three different haustorial and protrusion specimens, respectively, are presented. n: number of specimens.

Detection of nuclei and callose-rich sieve plates in green fluorescent protein (GFP)-conducting cells in a 1-cm-diameter tubercle. (A–C) 4′6-diamidino-2-phenylindole (DAPI) staining of haustorial cells; (A) GFP, (B) DAPI, and (C) merged. (D–F) DAPI staining of protrusion cells; (D) GFP, (E) DAPI, and (F) merged. White arrows indicate nuclei. (G–I) Aniline Blue staining of haustorial cells; (G) GFP, (H) Aniline Blue, and (I) merged. (J–L) Aniline Blue staining of protrusion cells; (J) GFP, (H) Aniline Blue, and (I) merged. White triangles indicate sieve plates. Scale bar: 50 μm. (M) Percentage of nucleated GFP-conducting cells to all GFP-conducting cells. Ha, haustorium; Pr, protrusion. The means and standard deviations of six and three different haustorial and protrusion specimens, respectively, are presented. n: number of specimens.