Table 1.
Relative mRNA transcripts for each cyp isoform in C57B/6 mice microsomes according to diet group.
| Tissue | Diet | cyp2b9 | cyp2b10 | cyp2c29 | cyp2c37 | cyp2c39 | cyp2c40 | cyp2d9 | cyp2d22 | cyp2e1 | cyp2j5 | cyp2j6 | cyp3a11 | cyp3a13 | cyp3a25 | cyp4a10 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Liver | ND | 0.01 | 0.02 | 0.49 | 1.31 | 0.01 | 0.44 | 0.55 | 0.53 | 0.56 | 0.98 | 1.08 | 0.58 | 2.40 | 0.44 | 1.25 |
| LDR | 4.89 * | NQ | 0.25 * | 1.77 | 0.01 | 0.20 | 0.81 | 0.59 | 1.02 | 1.05 | 0.88 | 0.04 ᵵ | 3.07 | 0.17 | 1.22 | |
| HDR | 8.51 ᵵ | 0.03 | 0.30 | 2.15 | 0.02 ** | 0.37 | 0.71 | 1.03 | 0.79 | 0.90 | 1.09 | 0.04 ᵵ | 2.96 | 0.21 | 3.72 | |
| Kidney | ND | ND | 0.54 | 2.35 | ND | ND | 8.62 | 1.65 | 1.32 | 1.28 | 4.89 | 4.56 | 1.41 | 1.37 | 2.00 | 1.42 |
| LDR | ND | 0.46 | 3.08 | ND | ND | 3.08 | 1.35 | 1.38 | 1.29 | 4.68 | 4.62 | 0.57 | 2.12 | 1.79 | 1.13 | |
| HDR | ND | 0.42 | 1.59 | ND | ND | 5.92 | 1.65 | 1.50 | 1.29 | 4.18 | 4.03 | 0.72 | 2.41 | 0.95 | 2.43 | |
| Heart | ND | ND | 1.21 | 0.26 | ND | ND | ND | 0.05 | 3.24 | 0.96 | 0.09 | 2.19 | ND | NQ | ND | 1.09 |
| LDR | ND | 2.16 | 0.49 | ND | ND | ND | 0.07 | 3.40 | 0.62 | 0.19 | 1.85 | ND | NQ | ND | 2.15 | |
| HDR | ND | 1.68 | 0.30 | ND | ND | ND | 0.07 | 5.03 | 0.51 * | 0.19 | 1.67 | ND | NQ | NQ | 1.47 | |
| Lung | ND | ND | 0.73 | 1.11 | ND | ND | ND | 0.07 | 2.06 | 0.21 | 0.13 | 1.20 | ND | 3.26 | 0.06 | 3.28 |
| LDR | ND | 1.66 ᵵ | 2.96 | ND | ND | ND | 0.08 | 2.05 | 0.33 ** | 0.47 | 1.88 | ND | 8.15 ** | 0.07 | 9.90 * | |
| HDR | ND | 1.17 ** | 1.00 | ND | ND | ND | ND | 5.76 | 0.32 * | 0.83 | 1.69 | ND | 5.39 | 0.11 | 3.43 |
Results are expressed as mean N-fold differences in cyp gene relative to the average expression of housekeeping genes and a calibrator (2−ΔΔCt sample). ND, Normal diet; LDR, low-diet responders; and HDR, high-diet responders. NQ = Not Quantifiable (35 < Ct < 38), ND = Not Detectable (Ct > 38). Each experiment was performed three times and in triplicates. One-way ANOVA was performed with Dunnett post-hoc test. LDR or HDR versus ND; * p < 0.05, ** p < 0.01, ᵵ p < 0.001.