Fig. 1. (A) Phylogenetic analysis for Rnf152 in higher vertebrates.

Neighbor-joining method of MEGA7 was applied to derive the phylogenetic tree upon the amino acid sequences of human (NP_775828), chimpanzee (XP_001143948), mouse (NP_848894), chicken (NP_001291963), Xenopus (XP_004915369) and zebrafish (NP_001014380) which were collected from NCBI database. The scale bar denotes the branch distance in the figure. (B) RT-PCR analysis for temporal expression pattern of zebrafish rnf152. rnf152 was expressed both maternally and zygotically in zebrafish embryos. β-actin (500bp) was served as internal control (lower panel). Early and late stages of the zebrafish embryos abundantly expressed rnf152. Amplicon size of rnf152 was 235bp and equal amount of cDNA was in each reaction of all the tested stages with the endogenous control [No. of experiments (n) =3].