Fig. 7.
Functional effects of OGFr overexpression on HCC cells. A, HepG2 and Huh-7 cells were transfected with empty vector (EV) or OGFr overexpression vector (pOGFr) for 48 h. OGFr overexpression efficiency was determined by qRT-PCR. The expression level of OGFr was normalized to GAPDH. B, Western blot analysis of OGFr protein expression 48 h after transfection with EV (pCMV-HA) or pOGFr (pCMV-HA-OGFr). GAPDH was used as an internal control. C, OGFr overexpression in HCC cells significantly inhibits cell growth. D, Effect of OGFr overexpression on HCC cell cycle. E, Effect of OGFr overexpression on cell invasion, as determined in a Boyden chamber assay. F, Relative cell invasion on the underside of the filter. Significantly enhanced invasion (p < 0.05) is indicated. G, OGFr overexpression had no effect on HCC cell migration as determined by a wound-healing assay. H, Quantification of the wound healing assay. I, Western blot showing the expression levels of p16INK4a and p21WAF1/CIP1 after OGFr overexpression or HOTAIR inhibition. Data are presented as means ± S.D. and represent results from three independent experiments. Statistically significant differences are indicated: *p < 0.05; **p < 0.01.