Fig. 2.

Proteomics of gonads from 11.5 to 13.5 dpc and global proteomics analyses. A, Schematic of the experimental design for proteomics, with assay results for gonads from 11.5 to 13.5 dpc. B, Peptides corresponding to 3666 proteins. C, Principal components analysis (PCA) of 11.5, 12.5, and 13.5 dpc. For each stage, there were 4 replicates. D, Cluster analysis of the 473 DE proteins, grouped into four clusters (C1, C2, C3, and C4). For each stage, there were 4 replicates. E, Unbiased hierarchical clustering of DE protein expression.