Fig. 7.

Rotenone can lead to germ cell cycle arrest, but CoQ10 can rescue this phenotype. A, Relative expression of p53, p15 and p21 in normal, control, 0.1 μm rotenone and 0.1 μm rotenone plus 20 μm CoQ10. B, Immunofluorescence results showing pRB1-positive germ cells clearly decreased after treatment with 0.1 μm rotenone, compared with in normal and control groups; addition of CoQ10 rescued the phenotype. C, Immunofluorescence also showed that cyclin E-positive germ cells were decreased in the rotenone treated group, compared with in normal and control groups, and CoQ10 could partially rescue this phenotype. Cyclin E-positive somatic cells were not detectably changed in the rotenone-treated group. Error bars indicate S.E. and similar experiments were each repeated at least three times (*p < 0.05, **p < 0.01, Student's t test). Bar, 50 μm.