Table I. Candidate surface antigens prioritized for further validation. Shown are the eleven candidate proteins with key features derived from PlasmoDB, including number of transmembrane domains (TM), presence of a signal peptide (SP) and an export motif. We also included previously reported localization, isolation method by which the candidate was identified, and genetic diversity. Proteins validated for surface expression are marked in bold.
| Accession ID | TM | SP | Export motif | Protein description | Previously described localizations | Detection method | Polymorphism level (πN) |
|---|---|---|---|---|---|---|---|
| PF3D7_0112900 | 2 | YES | YES | Plasmodium exported protein | Supernatant | 0.00049 | |
| PF3D7_0220000 | 2 | YES | YES | LSA3 | Membranes | 0.0039 | |
| PF3D7_0501200 | 3 | YES | YES | PIESP2 | MC (21), Surface (20) | Membranes, Supernatant | 0.0016 |
| PF3D7_0619800 | 6 | NO | NO | Conserved Plasmodium membrane protein | Supernatant | 0.0014 | |
| PF3D7_0820400 | 2 | NO | NO | Conserved Plasmodium protein | Supernatant | 0.00040 | |
| PF3D7_1001900 | 3 | YES | YES | PfJ23 | MC (21) | Membranes/Supernatant | 0.000096 |
| PF3D7_1024800 | 2 | YES | NO | Conserved Plasmodium protein | Membranes | 0.0017 | |
| PF3D7_1249000 | 4 | NO | NO | Conserved Plasmodium membrane protein | Supernatant | 0.00053 | |
| PF3D7_1301700 | 3 | YES | YES | GEXP07 | Supernatant | 0.000061 | |
| PF3D7_1310500 | 2 | YES | NO | Conserved Plasmodium protein | Supernatant | 0.00040 | |
| PF3D7_1439300 | 3 | YES | NO | Conserved Plasmodium protein | Supernatant | 0.00055 |