Table 1.
S.no. | Genes | Primers | Function |
---|---|---|---|
1. | Fibrillin 1a |
FiB F:
5′-TTAGATGCTCGTGCGAATGG-3′ |
Involved in photoprotection against photoinhibition |
FiB R:
5′-CGCTATACTTGGACGAACCTTG-3′ |
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2. | GmDREB1B |
GmDREB1B F:
5′-GTAAAGATTGTTCGTATGGGACAAG-3′ |
Drougt tolerance by regulating expression of GmPLY21 |
GmDREB1B R:
5′-ACACCTAAAATGAGCAACCGTACTA-3′ |
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3. | GmTP55 (Antiquitin) |
GmTP55 F:
5′-CGAAAAGGGAGAGGAGGACTTC-3′ |
Aldehyde dehydrogenase gene involved in drought tolerance |
GmTP55 R:
5′-TCTGGGTCACCGAAAGGCAA-3′ |
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4. | GmBIP |
GmBIPD F:
5′-ATCTGGAGGAGCCCCAGGCGGTGG-3′ |
BiP overexpression confers resistance to drought |
GmBIPD R:
5′-CTTGAAGAAGCTTCGTCGTAAAACTAAG-3′ |
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5. | GmGST |
GmGST F:
5′-CGGTTCTCATCCACAATGGCAAAC-3′ |
Drought tolerance; Glutathione S transferase |
GmGST R:
5′-CAGCCCAGAATCTAGCCTGAGC-3′ |
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6. | GmRD22 |
GmRD22 F:
5′-AATGCCGAAAGCCATTACAG-3′ |
Drought tolerance |
GmRD22 R:
5′-GCTTTGTTTTCCCTGCGTTA-3′ |
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7. | GmNFYA3 |
GmNFYA3 F:
5′-ACCAGAATTGCATTACCAGTTGA-3′ |
Induced by ABA and drought; is a positive regulator of drought tolerance |
GmNFYA3 R:
5′-GGTGCCGAGACTCATGAAGATAT-3′ | |||
8. | GmPIP1b |
GmPIP1b F:
5′-TCATGGGTTTCAAAAAGGAGA-3′ |
Aquaporins |
GmPIP1b R:
5′-GCTTGCAATAAAAGCACAAGC-3′ | |||
9. | GmCYP707A1a |
GmCYP707A1a F:
5′-GGAAGATGATTGATTACAAGGAC-3′ |
ABA 8′-hydroxylase induced by dehydration |
GmCYP707A1a R:
5′-GACACTGGGGTTTTCACCGA-3′ | |||
10. | GmCYP707A3b |
GmCYP707A3b F: 5′-GGCTAACCTTCTGACTTTCC-3′ |
ABA 8′-hydroxylase induced by rehydration |
GmCYP707A3b R: 5′-CAAGTGTCTGGTTCTGAGGT-3′ | |||
11. | GmERD1 |
GmERD1 F:
5′-CGTCCAGAATTGCTCAACAG-3′ |
Dehydration induced |
GmERD1 R:
5′-TGGGGTTATAGCCTTGTTGG-3′ | |||
12. | GmRD20 |
GmRD20 F:
5′-GTGGCACATGACTGAAGGAA-3′ |
Involved in dehydration |
GmRD20 R:
5′-ATCTTTCCAGCAGCACCTCT-3′ | |||
13. | Tubulin |
Tubulin F:
5′-ATGTTCCGTGGCAAGATGAG-3′ |
Housekeeping gene for reference |
Tubulin R:
5′-CATTGTTGGGAATCCACTCG-3′ |