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. 2017 Oct 11;10(1):plx051. doi: 10.1093/aobpla/plx051

Table 1.

List of the primers used in gene expression analysis.

S.no. Genes Primers Function
1. Fibrillin 1a FiB F:
5′-TTAGATGCTCGTGCGAATGG-3′
Involved in photoprotection against photoinhibition
FiB R:
5′-CGCTATACTTGGACGAACCTTG-3′
2. GmDREB1B GmDREB1B F:
5′-GTAAAGATTGTTCGTATGGGACAAG-3′
Drougt tolerance by regulating expression of GmPLY21
GmDREB1B R:
5′-ACACCTAAAATGAGCAACCGTACTA-3′
3. GmTP55 (Antiquitin) GmTP55 F:
5′-CGAAAAGGGAGAGGAGGACTTC-3′
Aldehyde dehydrogenase gene involved in drought tolerance
GmTP55 R:
5′-TCTGGGTCACCGAAAGGCAA-3′
4. GmBIP GmBIPD F:
5′-ATCTGGAGGAGCCCCAGGCGGTGG-3′
BiP overexpression confers resistance to drought
GmBIPD R:
5′-CTTGAAGAAGCTTCGTCGTAAAACTAAG-3′
5. GmGST GmGST F:
5′-CGGTTCTCATCCACAATGGCAAAC-3′
Drought tolerance; Glutathione S transferase
GmGST R:
5′-CAGCCCAGAATCTAGCCTGAGC-3′
6. GmRD22 GmRD22 F:
5′-AATGCCGAAAGCCATTACAG-3′
Drought tolerance
GmRD22 R:
5′-GCTTTGTTTTCCCTGCGTTA-3′
7. GmNFYA3 GmNFYA3 F:
5′-ACCAGAATTGCATTACCAGTTGA-3′
Induced by ABA and drought; is a positive regulator of drought tolerance
GmNFYA3 R:
5′-GGTGCCGAGACTCATGAAGATAT-3′
8. GmPIP1b GmPIP1b F:
5′-TCATGGGTTTCAAAAAGGAGA-3′
Aquaporins
GmPIP1b R:
5′-GCTTGCAATAAAAGCACAAGC-3′
9. GmCYP707A1a GmCYP707A1a F:
5′-GGAAGATGATTGATTACAAGGAC-3′
ABA 8′-hydroxylase induced by dehydration
GmCYP707A1a R:
5′-GACACTGGGGTTTTCACCGA-3′
10. GmCYP707A3b GmCYP707A3b F:
5′-GGCTAACCTTCTGACTTTCC-3′
ABA 8′-hydroxylase induced by rehydration
GmCYP707A3b R:
5′-CAAGTGTCTGGTTCTGAGGT-3′
11. GmERD1 GmERD1 F:
5′-CGTCCAGAATTGCTCAACAG-3′
Dehydration induced
GmERD1 R:
5′-TGGGGTTATAGCCTTGTTGG-3′
12. GmRD20 GmRD20 F:
5′-GTGGCACATGACTGAAGGAA-3′
Involved in dehydration
GmRD20 R:
5′-ATCTTTCCAGCAGCACCTCT-3′
13. Tubulin Tubulin F:
5′-ATGTTCCGTGGCAAGATGAG-3′
Housekeeping gene for reference
Tubulin R:
5′-CATTGTTGGGAATCCACTCG-3′