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. 2017 Nov 24;18(12):2512. doi: 10.3390/ijms18122512

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(1) HPLC chromatogram of crude Hypericum laricifolium Juss. (HL) 70% MeOH extract at 210 nm and offline HPLC of (A) HL based on offline α-glucosidase UF-HPLC. (B) HL based on offline HRAR UF-HPLC. Antioxidants determined by (C) offline DPPH-HPLC and (D) offline ABTS-HPLC. (2) Total Binding Degree (%) and Quantitative Reduction (%) of (A) offline α-glucosidase UF-HPLC, (B) offline HRAR UF-HPLC, (C) offline DPPH-HPLC and (D) offline ABTS-HPLC. * DPPH peak; * ABTS peak. HPLC: High-Performance Liquid Chromatography; UF: Ultrafiltration; HRAR: Human recombinant aldose reductase; DPPH: 2,2-diphenyl-1-picrylhydrazyl; ABTS: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); MeOH: Methanol.