Figure 6.

The aptamer affinity maturation process. The yellow arrow designates the aptamer affinity maturation process, in which data from a SELEX experiment is utilized by bioinformatic software to Create a novel aptamer library which undergoes a microarray screening process. Sequence information and, if available, folding information (optional) from a SELEX experiment is used as input for the resample software. Resample outputs a novel focused aptamer library that is small enough to fit on a DNA microarray. The microarray is incubated with a fluorescently labeled target before washing and scanning to measure the binding strength of all aptamers. Figure adapted from Kinghorn et al. [118].