Figure 4.
The effect of GO-AgNPs treatment on differentiation of SH-SY5Y cells. The GO-AgNPs induced differentiation of SH-SY5Y cells was determined after 24-h exposure to GO (10 µg/mL), AgNPs (5 µg/mL) and GO-AgNPs (1.0 µg/mL). Phase contrast microscopy images showing the morphological changes in SH-SY5Y cells after treatment with GO-AgNPs in 1% serum-supplemented medium. The green arrows indicate significant, lengthy neurite outgrowth. At least three independent experiments were performed for each sample. Scale bar is 100 μm.