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. 2017 Nov 28;18(12):2549. doi: 10.3390/ijms18122549

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of expression of various neuronal markers. Microtubule-associated protein 2 (MAP2), synaptophysin, neurofascin (NFASC), protein kinase C, alpha (PRKCA), β tublin III, fox-1 homolog 3 (NEUN), growth associated protein 43 (GAP-43) and neurogenin 1, were analyzed after exposure of SH-SY5Y cells to GO (10 µg/mL), AgNPs (5 µg/mL) and GO-AgNPs (1.0 µg/mL) for 24 h. After 24 h treatment expression fold level was determined as fold changes in reference to expression values against GAPDH; (B) Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of expression of various neuronal markers. The expression pattern of various neuronal markers genes such as dopamine receptors type 2 (DRD2), neuropilin 1 (NRP1), gamma neuronal (NSE), alkaline phosphatase (ALP), neuropeptide Y, (NPY), microtubule-associated protein tau (TAU), laminin, and collagen type IV were analyzed after exposure of SH-SY5Y cells to GO (10 µg/mL), AgNPs (5 µg/mL) and GO-AgNPs (1.0 µg/mL) for 24 h. After 24 h treatment expression fold level was determined as fold changes in reference to expression values against GAPDH. Results are expressed as fold changes. At least three independent experiments were performed for each sample. The results are expressed as the mean ± standard deviation of three independent experiments The treated groups showed statistically significant differences from the control group by the Student’s t-test (* p < 0.05).

(A) Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of expression of various neuronal markers. Microtubule-associated protein 2 (MAP2), synaptophysin, neurofascin (NFASC), protein kinase C, alpha (PRKCA), β tublin III, fox-1 homolog 3 (NEUN), growth associated protein 43 (GAP-43) and neurogenin 1, were analyzed after exposure of SH-SY5Y cells to GO (10 µg/mL), AgNPs (5 µg/mL) and GO-AgNPs (1.0 µg/mL) for 24 h. After 24 h treatment expression fold level was determined as fold changes in reference to expression values against GAPDH; (B) Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of expression of various neuronal markers. The expression pattern of various neuronal markers genes such as dopamine receptors type 2 (DRD2), neuropilin 1 (NRP1), gamma neuronal (NSE), alkaline phosphatase (ALP), neuropeptide Y, (NPY), microtubule-associated protein tau (TAU), laminin, and collagen type IV were analyzed after exposure of SH-SY5Y cells to GO (10 µg/mL), AgNPs (5 µg/mL) and GO-AgNPs (1.0 µg/mL) for 24 h. After 24 h treatment expression fold level was determined as fold changes in reference to expression values against GAPDH. Results are expressed as fold changes. At least three independent experiments were performed for each sample. The results are expressed as the mean ± standard deviation of three independent experiments The treated groups showed statistically significant differences from the control group by the Student’s t-test (* p < 0.05).