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. 2017 Nov 28;18(12):2552. doi: 10.3390/ijms18122552

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Extraction protocols and representative miRNA yield from neonatal screening cards (NSC). (A) Flow diagram of 4 out of 11 extraction protocols performed. (B) Gel-like image for small RNA quality from 11 protocols for miRNA purification performed from dried blood samples on NSC. (C) Electropherogram from protocols 3 (purple), 4 (orange), 7 (magenta) and 8 (gold). (D) miRNA:small RNA ratio from NSC of normal (green), low body weight (red) and macrosomia (blue) neonates, horizontal line indicates the 0.35 threshold ratio, each bar represents an individual, n = 20. NBW, normal birth weight; LBM, low birth weight.