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. 2017 Nov 29;18(12):2565. doi: 10.3390/ijms18122565

Figure 1.

Figure 1

DNA double-strand break repair pathways. (A) Homology dependent repair, applicable in the presence of a homologous repair template (green) and error-free non-homologous end joining pathway. The later, however, is responsible for the regeneration of the target site through religation of the paired end complex. Red lightning symbol indicates introduction of a DSB. Large red arrow highlights efficient repair and regeneration of the nuclease target site by NHEJ. (B) Schematic model representing how TevCas9 can bias DNA repair outcome (see text for details). Red dashed rectangles, endonuclease target site; green dashed rectangles, mutated target site after error-prone repair. NHEJ, non-homologous end joining; HDR, homology directed repair; cNHEJ, classical NHEJ; alt-NHEJ, alternative-NHEJ.