Table 8.
Malondialdehyde (MDA) content, superoxide dismutase (SOD) and catalase activities of D. palmatum leaves and antioxidant activity of D. palmatum extracts obtained from the herb grown under different temperatures of cultivation and luteolin-7-O-glucoside as a reference compound a,b.
| Parameter | Luteolin-7-O-Glucoside | Temperature (°C) | |
|---|---|---|---|
| 20 | 1 | ||
| MDA content (nM/g) FW | - | 92.74 ± 7.41 | 197.02 ± 15.76 |
| SOD activity (U/g·min) FW | - | 57.90 ± 5.21 | 264.32 ± 12.35 |
| Catalase activity (U/g·min) FW | - | 0.93 ± 0.06 | 1.53 ± 0.12 |
| Total antioxidant capacity (mg-eq). luteolin-7-O-glucoside/g | 1000 iii | 280.98 ± 8.99 i | 682.26 ± 21.15 ii,iii |
| DPPH•-radical scavenging activity, IC50 (µg/mL) | 16.97 ± 0.34 iv,v | 33.28 ± 0.73 vi | 11.40 ± 0.24 iv |
| ABTS•+-radical scavenging activity, IC50 (µg/mL) | 9.86 ± 0.19 vii,viii | 14.62 ± 0.31 viii | 5.69 ± 0.11 vii |
| O2•−-radical scavenging activity, IC50 (µg/mL) | 14.92 ± 0.43 ix,x | 18.36 ± 0.56 x | 9.21 ± 0.21 ix |
| Br•-radical scavenging activity (mg-eq). luteolin-7-O-glucoside/g | 1000 xii | 150.19 ± 1.95 xi | 799.63 ± 11.19 xii |
| NO inactivating activity, IC50 (µg/mL) | >100 | 37.92 ± 1.59 | 21.37 ± 0.85 xiii |
| H2O2 inactivating activity (mM/g) | 0.53 ± 0.02 xiv | 1.56 ± 0.04 | 2.75 ± 0.06 xv |
| Fe2+-chelating activity (µM) Fe2+/g | 106.12 ± 3.18 xvi | 142.84 ± 4.42 xvi,xvii | 206.11 ± 4.78 xviii |
a Averages ± standard deviations (SD) were obtained from five different experiments; b Values with different roman letters (i–xviii) indicate statistically significant differences among groups at p < 0.05 by one-way analysis of variance.