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. 2017 Dec 1;18(12):2581. doi: 10.3390/ijms18122581

Figure 1.

Figure 1

RNF7 binds to CARMA2sh. (A) HEK293T cells were cotransfected with a plasmid encoding for HA-tagged RNF7 together with a FLAG-tagged expression vector empty or encoding for CARMA2sh. Twenty-four hours later, lysates were immunoprecipitated with anti-FLAG antibodies and analyzed for coprecipitating HA-RNF7 by Western blot assay. (B) HaCaT cells were left untreated or stimulated with IL-1β for 30 min. Cell lysates where then prepared, immunoprecipitated with anti-RNF7 or anti-myc control antibodies, and analyzed for coprecipitating CARMAsh by Western blot assay. IP: Immunoprecipitation; WB: Western Blot.