Figure 5.

RNF7 controls the ubiquitination state of NF-kappa-B essential modulator (NEMO) and Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1). HEK293T cells were cotransfected with the indicated expression vectors. Twenty-four hours later, lysates were prepared and immunoprecipitated with (A) anti-BCL10, (B) anti-HA, (C) anti-MALT1, and (D) anti-NEMO antibodies. Immunocomplexes were separated by SDS-PAGE and blotted on membranes subsequently probed with anti-ubiquitin antibody.