Table 1.
Interaction of CARMA2short (CARMA2sh) with RING finger protein 7 (RNF7) in the yeast two-hybrid assay.
| Protein Fused to GAL4 Domain | Yeast Growth on Selective Media | |
|---|---|---|
| DNA-Binding | Activating | |
| - | RNF7 | − |
| Vector | RNF7 | − |
| Fas-associated protein with death domain (FADD) | RNF7 | − |
| CARMA2sh | RNF7 | + |
| CARMA2sh | - | − |
Yeast AH109 was transformed with CARMA2sh fused to the GAL4-DNA binding domain together with the indicated cDNAs fused to the GAL4-activating domain. The cDNA encoding for FADD served as a putative negative control. Interactions were examined by yeast growth on selective media; assays were done for 10 independent transformants. Yeast colonies were scored as positive (+) when growth developed within 24–36 h; a negative (−) was scored when growth failed to develop within 1 week.