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Effect of siRNA-mediated Slc1a1 knockdown on GSH and GSSG levels. PCNs were transfected with either nontargeting scramble siRNA or a SMARTpool mix of four siSlc1a1 using siPORT amine. 48 h post-transfection, protein extracts were immunoblot-analyzed for EAAC1 (A) (n = 3); and fluorimetry-based determination of GSH and GSSG content (B). The bar graph represents the GSH/GSSG ratio with n = 2 pooled from 3 individual samples (B).
