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. 2017 Dec 5;18(12):2596. doi: 10.3390/ijms18122596

Figure 7.

Figure 7

Effect of in utero N-acetylcysteine (NAC) pretreatment on the levels of EAAC1 protein, Cys, and GSH content. Pregnant rats (Sprague-Dawley) at embryonic day 17 were administered NAC (100 mg/kg) 1 h prior to the 1st dose of E. In total, 5 doses of E (3.5 g/kg b.wt.) or isocaloric dextrose were administered by gastric intubation at 12-h intervals. At embryonic day 19 brain cortex from embryos were dissected and processed for HPLC-based determination of Cys concentration (n = 3) (A); GSH content (n = 3) (B); Expression of EAAC1 protein in fetal brain cortices by immunoblotting (top panel) and ImageJ quantification of EAAC1 normalized to GAPDH bands (bottom panel (n = 3) (C). Values represent the mean ± SEM. * p < 0.05 was considered significant for ethanol alone; ns—not significant versus NAC alone treatment.