Figure 14.

The difference in insulin (INS) binding to glycated and unglycated insulin receptor (IR) in PBS with and without 20 mM glucose. Note that although the binding constants of INS binding under these conditions differed (see Figure 12 and Figure 13), the effect of glycation on decreasing INS binding was the same. Thus, the diminution in INS binding to glycated IR is independent of the presence or absence of free glucose.