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. 2017 Dec 2;18(12):2603. doi: 10.3390/ijms18122603

Figure 1.

Figure 1

Quinacrine inhibits cell-surface intercellular adhesion molecule-1 (ICAM-1) protein expression induced by TNF-α and IL-1α. (a) A549 cells were preincubated with various concentrations of quinacrine for 1 h and then incubated with TNF-α (2.5 ng/mL) or IL-1α (0.25 ng/mL) or without (control) cytokines, in the presence or absence of quinacrine, for 6 h. ICAM-1 expression in TNF-α-stimulated cells and IL-1α-stimulated cells without quinacrine was set to 100%. ICAM-1 expression in unstimulated cells was calculated based on TNF-α-stimulated cells without quinacrine. Data are shown as the mean ± SE of three independent experiments. ** p < 0.01 and *** p < 0.001 indicate significant differences from the control. (b) A549 cells were preincubated with various concentrations of quinacrine for 1 h and then incubated with TNF-α (2.5 ng/mL) or IL-1α (0.25 ng/mL) or without (control) cytokines, in the presence or absence of quinacrine, for 6 h. Cell viability was evaluated by the MTT assay. MTT reduction was calculated based on unstimulated cells without quinacrine as 100%. MTT reduction (%) is shown as the mean ± SE of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 indicate significant differences from the control.