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. 2017 Dec 2;18(12):2603. doi: 10.3390/ijms18122603

Figure 2.

Figure 2

Quinacrine inhibits ICAM-1 mRNA expression induced by tumor necrosis factor (TNF)-α and interleukin 1 (IL-1)α. (a) A549 cells were preincubated with various concentrations of quinacrine for 1 h and then incubated with TNF-α (2.5 ng/mL) or IL-1α (0.25 ng/mL) or without (control) cytokines, in the presence or absence of quinacrine, for 2 h. ICAM-1 mRNA expression was analyzed by quantitative-PCR. ICAM-1 mRNA (fold) is shown as the mean ± SE of three independent experiments. *** p < 0.001 indicates significant differences from the TNF-α or IL-1α stimulation. (b) A549 cells were preincubated with various concentrations of quinacrine for 1 h and then incubated with TNF-α (2.5 ng/mL) or IL-1α (0.25 ng/mL) or without (control) cytokines, in the presence or absence of quinacrine, for 2.5 h. Cell viability was evaluated by the MTT assay. MTT reduction (%) is shown as the mean ± SE of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 indicate significant differences from the control.