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. 2017 Dec 2;18(12):2603. doi: 10.3390/ijms18122603

Figure 4.

Figure 4

Quinacrine inhibits TNF-α-induced ICAM-1 expression and NF-κB-responsive luciferase activity in other cancer cell lines. (a) MCF-7 cells were preincubated with various concentrations of quinacrine for 1 h and then incubated with TNF-α (2.5 ng/mL) or without (control) cytokines, in the presence or absence of quinacrine, for 6 h. ICAM-1 expression in TNF-α-stimulated cells without quinacrine was set to 100%. Data are shown as the mean ± SE of three independent experiments. *** p < 0.001 indicates significant differences from the control. (b) MCF-7 cells were preincubated with various concentrations of quinacrine for 1 h and then incubated with TNF-α (2.5 ng/mL) or without (control) cytokines, in the presence or absence of quinacrine, for 6 h. Cell viability was evaluated by the MTT assay. MTT reductions were calculated based on unstimulated cells without quinacrine as 100%. MTT reduction (%) is shown as the means ± SE of three independent experiments. ** p < 0.01 and *** p < 0.001 indicate significantly differences from the control. (c,d) MCF-7 cells (c) and HT-1080 cells (d) were transiently transfected with the NF-κB-responsive firefly luciferase reporter and cytomegalovirus promoter-driven Renilla luciferase reporter for 24 h. MCF-7 cells and HT-1080 cells were preincubated with various concentrations of quinacrine for 1 h and then incubated with TNF-α (2.5 ng/mL) or without cytokines (–), in the presence or absence of quinacrine, for 2.5 h. Luciferase activity (fold) is shown as the mean ± SE of seven (c) and six (d) independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 indicate significant differences from the TNF-α stimulation without quinacrine. (e,f) MCF-7 cells (e) and HT-1080 cells (f) were preincubated with various concentrations of quinacrine for 1 h and then incubated with TNF-α (2.5 ng/mL) or without (control) cytokines, in the presence or absence of quinacrine, for 2.5 h. Cell viability was evaluated by the MTT assay. MTT reduction (%) is shown as the means ± SE of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 indicate significant differences from the control.