. 2017 Dec 4;18(12):2607. doi: 10.3390/ijms18122607

Table 5.

Comparison of EPA:DHA mixtures on cell viability in human BC cell lines.

Citation	Cell Line	Concentration of EPA or DHA (µM)	Ratio EPA:DHA	Assay	Change in Cell Viability *	Exposure (Hours)	Form of n-3 LCPUFA
Schley, Jijon, Robinson and Field [29]	MDA-MB-231	60 EPA + 40 DHA	1.5:1	TBE	↓40%	72	Dissolved in ethanol
Schley, Jijon, Robinson and Field [29]	MDA-MB-231	45 EPA + 30 DHA + 75 LA	1.5:1	TBE	↓31%	72	Dissolved in ethanol
Schley, Brindley and Field [13]	MDA-MB-231	60 EPA + 40 DHA	1.5:1	TBE	↓62%	72	Dissolved in ethanol
Schley, Brindley and Field [13]	MDA-MB-231	45 EPA + 30 DHA + 75 LA	1.5:1	TBE	↓48%	72	Dissolved in ethanol
Mansara, Deshpande, Vaidya and Kaul-Ghanekar [32]	MDA-MB-231	84–153 EPA + 56–102 DHA + 25–140 AA	1.5:1	TBE	↓54%–↓82%	24	Conjugated to delipidated, endotoxin free BSA
	MDA-MB-231	84–153 EPA + 56–102 DHA + 25–140 AA	1.5:1	MTT	↓15%–↓30%	24	Conjugated to delipidated, endotoxin free BSA
	MCF-7	84–153 EPA + 56–102 DHA + 25–140 AA	1.5:1	TBE	↓38%–↓81%	24	Conjugated to delipidated, endotoxin free BSA
	MCF-7	84–153 EPA + 56–102 DHA + 25–140 AA	1.5:1	MTT	↓20%–↓30%	24	Conjugated to delipidated, endotoxin free BSA

EPA = eicosapentaenoic acid, DHA = docosahexaenoic acid; AA = arachidonic acid; TBE = Trypan Blue Exclusion; MTT = 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; “↓” denotes significant decrease (p < 0.05); “%” = percent change from control condition; BSA = bovine serum albumin.; * Relative to control conditions.