Figure 5.

doxorubicin (DOX) inhibits activation of IRE-1α and XBP-1. In basic DMEM, cells were treated with DMSO and 1.0 μg mL⁻¹ TUNI or a combination of TUNI and DOX for 24 h. (A) Expression of IRE-1α and XBP-1s proteins was determined using the Western blot; one representative protein band from the three independent experiments is shown; (B) Relative abundance of IRE-1α; (C) Relative abundance of XBP-1s; (D) Relative cell viability was measured using CCK-8 after the treatment. β-actin was assessed as a loading control. Data given as means ± SDs for the three independent experiments (* means under T test, p < 0.05, ** means under T test, p < 0.01).