Figure 6.

Inhibition of IRE-1α and XBP-1 reverses the anti-apoptotic effect of l-glutamine. In basic DMEM, cells were treated with 1.0 μg mL⁻¹ TUNI and 0.45 g L⁻¹ l-glutamine or a combination of TUNI, l-glutamine and 4 μM DOX for 24 h. (A) Expression of GADD153, IRE-1α, XBP-1s and GRP78 proteins was measured using the Western blot; two representative protein bands from the three independent experiments are shown; (B) Relative abundance of GADD153; (C) Relative abundance of cleaved caspase-3; (D) Relative abundance of XBP-1s; (E) Relative abundance of IRE-1α; (F) Relative abundance of GRP78; (G) Relative cell viability was measured using CCK-8 after treatment. β-actin was assessed as a loading control, and data are given as the means ± SDs for the three independent experiments (* means under T test, p < 0.05, ** means under T test, p < 0.01).