Table A1.
Thirty-two types of N-glycans that showed good quantitative reproducibility in all Igs fraction samples and could be analyzed statistically.
| # | m/z | Composition |
|---|---|---|
| 1 | 1362.5 | (Hex)2 + (Man)3(GlcNAc)2 |
| 2 | 1524.5 | (Hex)3 + (Man)3(GlcNAc)2 |
| 3 | 1565.5 | (Hex)5 + (HexNAc)3 |
| 4 | 1590.6 | (HexNAc)2(dHex)1 + (Man)3(GlcNAc)2 |
| 5 | 1606.6 | (Hex)1(HexNAc)2 + (Man)3(GlcNAc)2 |
| 6 | 1647.6 | (HexNAc)3 + (Man)3(GlcNAc)2 |
| 7 | 1686.6 | (Hex)4 + (Man)3(GlcNAc)2 |
| 8 | 1708.6 | (Hex)1(HexNAc)1(NeuAc)1 + (Man)3(GlcNAc)2 |
| 9 | 1752.6 | (Hex)1(HexNAc)2(dHex)1 + (Man)3(GlcNAc)2 |
| 10 | 1768.6 | (Hex)2(HexNAc)2 + (Man)3(GlcNAc)2 |
| 11 | 1793.7 | (HexNAc)3(dHex)1 + (Man)3(GlcNAc)2 |
| 12 | 1809.7 | (Hex)1(HexNAc)3 + (Man)3(GlcNAc)2 |
| 13 | 1848.6 | (Hex)5 + (Man)3(GlcNAc)2 |
| 14 | 1870.7 | (Hex)2(HexNAc)1(NeuAc)1 + (Man)3(GlcNAc)2 |
| 15 | 1914.7 | (Hex)2(HexNAc)2(dHex)1 + (Man)3(GlcNAc)2 |
| 16 | 1955.7 | (Hex)1(HexNAc)3(dHex)1 + (Man)3(GlcNAc)2 |
| 17 | 2010.7 | (Hex)6 + (Man)3(GlcNAc)2 |
| 18 | 2032.7 | (Hex)3(HexNac)1(NeuAc)1 + (Man)3(GlcNAc)2 |
| 19 | 2057.8 | (Hex)1(HexNAc)2(dHex)1(NeuAc)1+ (Man)3(GlcNAc)2 |
| 20 | 2073.8 | (Hex)2(HexNAc)2(NeuAc)1+ (Man)3(GlcNAc)2 |
| 21 | 2117.8 | (Hex)2(HexNAc)3(dHex)1 + (Man)3(GlcNAc)2 |
| 22 | 2219.8 | (Hex)2(HexNAc)2(dHex)1(NeuAc)1 + (Man)3(GlcNAc)2 |
| 23 | 2336.9 | (Hex)3(HexNAc)4 + (Man)3(GlcNAc)2 |
| IS | 2348.9 1 | Internal standard (BOA-labeled A2 amide) |
| 24 | 2378.9 | (Hex)2(HexNAc)2(NeuAc)2 + (Man)3(GlcNAc)2 |
| 25 | 2422.9 | (Hex)2(HexNAc)3(dHex)1(NeuAc)1 + (Man)3(GlcNAc)2 |
| 26 | 2524.9 | (Hex)2(HexNAc)2(dHex)1(NeuAc)2 + (Man)3(GlcNAc)2 |
| 27 | 2727.9 | (Hex)2(HexNAc)3(dHex)1(NeuAc)2 + (Man)3(GlcNAc)2 |
| 28 | 2743.9 | (Hex)3(HexNAc)3(NeuAc)2 + (Man)3(GlcNAc)2 |
| 29 | 2890.1 | (Hex)3(HexNAc)3(dHex)1(NeuAc)2 + (Man)3(GlcNAc)2 |
| 30 | 3049.1 | (Hex)3(HexNAc)3(NeuAc)3 + (Man)3(GlcNAc)2 |
| 31 | 3195.2 | (Hex)3(HexNAc)3(dHex)1(NeuAc)3 + (Man)3(GlcNAc)2 |
| 32 | 3341.2 | (Hex)3 (HexNAc)3 (Deoxyhexose)2 (NeuAc)3 + (Man)3(GlcNAc)2 |
1 m/z 2348.9 is the internal standard, disialo-galactosylated biantennary N-glycan, which contains amidated sialic acid residues (A2 amide glycans). Compositional annotations and putative structures are represented by the following abbreviations. Hex: hexose, HexNAc: N-acetylhexosamine, dHex: deoxyhexose.