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. 2017 Dec 13;18(12):2690. doi: 10.3390/ijms18122690

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of MEV, GGpp and Fpp on the simvastatin-increased p21^cip and p27^kip expression in ATC cells. (A) Simvastatin (10 μM) increased the mRNA expression level of p21^cip. Values represent the means ± SEM (n = 3). * p < 0.05, different from corresponding control. Simvastatin increased the protein levels of p21^cip and p27^kip and decreased the protein levels of CCND1 (B), hyperphosphorylated-Rb and E2F1 (C). These effects were prevented by MEV (50 μM) or GGpp (20 μM), but not Fpp (20 μM). SW1736 and 8305C cells were incubated with simvastatin alone or in the presence of MEV, GGpp or Fpp for 48 h, and then the mRNA and protein levels of p21^cip and p27^kip were quantified and analyzed. GAPDH or β-actin proteins served as control for equivalent loading. Values shown in parentheses represent the quantified results adjusted with corresponding GAPDH or β-actin protein level and expressed as ratio over control. C, control; Veh, simvastatin-incubated group.

Effects of MEV, GGpp and Fpp on the simvastatin-increased p21^cip and p27^kip expression in ATC cells. (A) Simvastatin (10 μM) increased the mRNA expression level of p21^cip. Values represent the means ± SEM (n = 3). * p < 0.05, different from corresponding control. Simvastatin increased the protein levels of p21^cip and p27^kip and decreased the protein levels of CCND1 (B), hyperphosphorylated-Rb and E2F1 (C). These effects were prevented by MEV (50 μM) or GGpp (20 μM), but not Fpp (20 μM). SW1736 and 8305C cells were incubated with simvastatin alone or in the presence of MEV, GGpp or Fpp for 48 h, and then the mRNA and protein levels of p21^cip and p27^kip were quantified and analyzed. GAPDH or β-actin proteins served as control for equivalent loading. Values shown in parentheses represent the quantified results adjusted with corresponding GAPDH or β-actin protein level and expressed as ratio over control. C, control; Veh, simvastatin-incubated group.