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. 2017 Dec 13;18(12):2690. doi: 10.3390/ijms18122690

Figure 6.

Figure 6

Effects of simvastatin on the EGF-enhanced ATC cell migration. Simvastatin (10 μM) diminished the migrated cell number of SW1736 and 8305C cells treated with or without EGF and evaluated using scratch assay (A) or Transwell assay (B). For horizontal cell migration, cells were pre-incubated with simvastatin for 1 h followed by a creation of migration gap and EGF administration in culture medium containing 2% FBS for additional 7 or 11 h. Cells in the migration gap were counted. For vertical cell migration, cells were pre-incubated with simvastatin for 4 h and then seeded into upper chamber of transwells containing 0% FBS. After 24 h the migrated cells attracted by the culture medium containing 5% FBS with or without EGF on the side of lower chamber of Transwell were counted. Values represent the means ± SEM (n = 3). * p < 0.05, different from corresponding control. # p < 0.05, different from EGF-incubated only group. C, control.