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. 2017 Dec 13;18(12):2699. doi: 10.3390/ijms18122699

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Chondrogenic differentiation in hASC and DFAT in the presence (CM: chondrogenic medium) or absence (GM: growth medium) of chondrogenic factors. (A) Microscopic evaluation of acid mucopolysaccharides in hASC (a) and DFAT (b) cells and their respective control (inset) by Alcian Blue 8GX stain at 30 days. Bars, 100 µm; (B) expression of ACAN and SOX9 of DFAT and hASC cells after 30 days in culture. Data are expressed as fold change of cells in a chondrogenic medium versus a growth medium.

Chondrogenic differentiation in hASC and DFAT in the presence (CM: chondrogenic medium) or absence (GM: growth medium) of chondrogenic factors. (A) Microscopic evaluation of acid mucopolysaccharides in hASC (a) and DFAT (b) cells and their respective control (inset) by Alcian Blue 8GX stain at 30 days. Bars, 100 µm; (B) expression of ACAN and SOX9 of DFAT and hASC cells after 30 days in culture. Data are expressed as fold change of cells in a chondrogenic medium versus a growth medium.