Table 4.

Cycling conditions for osteogenic, adipogenic, and chondrogenic differentiation. (min, minutes and s, seconds).

Cycling Conditions
Lineage	Initial Denaturation and Time	Cycles of Denaturation, Temperature and Time	Annealing Temperature and Time	Elongation Time
Osteogenic differentiation	95 °C—5 min	40 cycles—95 °C—30 s	58 °C—30 s	72 °C—40 s
Adipogenic differentiation	95 °C—5 min	40 cycles—95 °C—15 s	61 °C—30 s	72 °C—45 s
Chondrogenic differentiation	95 °C—5 min	40 cycles—95 °C—30 s	61 °C—30 s	72 °C—45 s