HFUS of the neck region of normal (A–C) and of orthotopic ATC bearing mouse (D–F). In (A): trans-axial brightness (B-)mode scan of the neck region at the level of the first tracheal rings; black arrows point to the normal thyroid lobes. In (B): longitudinal B-mode scan paramedian to the trachea; the yellow dotted line identifies a normal thyroid lobe. In (C): trans-axial color-Doppler scan of (A); the yellow arrow indicates the low blood flow of the normal thyroid. In red, blood flow towards the ultrasound beam, in blue, blood flow shifting away from it. In (D): trans-axial B-mode scan of the neck region at the level of the first tracheal rings; white arrow points to the normal thyroid lobe and the yellow dotted line identifies the orthotopic ATC two weeks after injection. In (E): longitudinal B-mode scan paramedian to the trachea; the yellow dotted line identifies the orthotopic ATC two weeks after injection. In (F): trans-axial color-Doppler scan of (D); the yellow arrow indicates the high blood flow of the orthotopic ATC two weeks after injection. In red, blood flow towards the ultrasound beam, in blue, blood flow shifting away from it.