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. 2017 Dec 16;18(12):2739. doi: 10.3390/ijms18122739

Figure 2.

Figure 2

Cytokine synthesis subject to stimulation and PUFA supplementation. Cytokine production was analyzed (A) on mRNA level by quantitative real-time PCR (N = 6, n = 3) and (B) on protein level by ELISA (N = 6, n = 2). TIME were cultured for 144 h in basic medium as well as in medium supplemented with 15 µmol/L α-linolenic acid (LNA, C18:3n3), eicosapentaenoic acid (EPA, C20:5n3), docosahexaenoic acid (DHA, C22:6n3), linoleic acid (LA, C18:2n6), and arachidonic acid (AA, C20:4n6), respectively. Stimulation was performed in the last 24 h of fatty acid supplementation by addition of the cytokines IL-1β, TNF-α, and IFN-γ (5 ng/mL each). Data are mean ± S.D. Superscript letters across a row denote significant differences.