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. 2017 Dec 19;18(12):2753. doi: 10.3390/ijms18122753

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MPP⁺ decreases Akt/GSK3β and ERK phosphorylation and increases p53 expression, whereas sulfuretin reverses its effect. SH-SY5Y cells were pretreated with sulfuretin for 2 h and then treated with MPP⁺ for 24 h. After cell lysis, the extracted proteins were subjected to Western blot analysis using specific antibodies. Protein levels of (A) p-Akt, Akt, p-GSK3β, GSK3β, p-CREB, and GAPDH; (B) p-ERK, ERK, p-p38, p38, p-JNK, and JNK were determined. Representative blots and their densitometric quantification are shown. Values are presented relative to control as mean fold change ± S.D. (n = 3). Differences are statistically significant at * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group and ## p < 0.01, and ### p < 0.001 vs. the MPP⁺ group.

MPP⁺ decreases Akt/GSK3β and ERK phosphorylation and increases p53 expression, whereas sulfuretin reverses its effect. SH-SY5Y cells were pretreated with sulfuretin for 2 h and then treated with MPP⁺ for 24 h. After cell lysis, the extracted proteins were subjected to Western blot analysis using specific antibodies. Protein levels of (A) p-Akt, Akt, p-GSK3β, GSK3β, p-CREB, and GAPDH; (B) p-ERK, ERK, p-p38, p38, p-JNK, and JNK were determined. Representative blots and their densitometric quantification are shown. Values are presented relative to control as mean fold change ± S.D. (n = 3). Differences are statistically significant at * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group and ## p < 0.01, and ### p < 0.001 vs. the MPP⁺ group.