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. 2017 Dec 19;18(12):2753. doi: 10.3390/ijms18122753

Figure 5.

Figure 5

Figure 5

LY294002 suppresses sulfuretin-induced protection against MPP+, whereas SB415286 reverses MPP+-induced cytotoxicity. SH-SY5Y cells were pretreated with or without LY294002 (10 μM) for 2 h, followed by treatment with or without sulfuretin (40 μM) for 2 h and exposed to MPP+ (1 mM) for 24 h. (A) Cell viability was measured by MTT assay. Values are presented relative to control as mean percentage change ± S.D. (n = 3). (B) Protein levels of p-Akt, Akt, p-GSK3β, GSK3β, p-ERK, ERK, and GAPDH were determined by Western blot analysis. Representative blots and their densitometric quantification are shown. Values are presented relative to control as mean fold change ± S.D. (n = 3). (C) SH-SY5Y cells were pretreated with or without SB415286 (20 μM) for 2 h, and then exposed to MPP+ (1 mM) for 24 h. Cell viability was measured by MTT assay. Values are presented relative to control as mean percentage change ± S.D. (n = 3). Differences are statistically significant at ** p < 0.01, *** p < 0.001 vs. the control group, ## p < 0.01, ### p < 0.001 vs. the MPP+ group, and $$ p < 0.01, $$$ p < 0.001 vs. the MPP+ and sulfuretin co-treated group.