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. 2017 Dec 19;18(12):2762. doi: 10.3390/ijms18122762

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transcript levels of TGF-β1, TGFβ-R1, SMAD3, p53 and RAS after treatment with 50 nM miR-34b mimic, NC mimic in PC3 cells by PCR (A) and qRT-PCR (B) β-actin was used as an internal control. The mRNA expression of genes was not significantly different in PC3 cells treated with or without miR-34b mimics. Per condition, three independent experiments were performed. TGF: transforming growth factor; SMAD: mothers against decapentaplegic; p53: a phosphorylated protein with a molecular weight of 53,000; RAS: rat sarcoma.